Anti-MMP-12; N-term (active)

Analysis Note

ControlConcentrated conditioned cell culture media collected from a proprietary human fibroblast cell line.

Application

Anti-MMP-12 Antibody, N-term (active) is an antibody against MMP-12 for use in WB.

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Matrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.

Immunogen

KLH-conjugated synthetic peptide corresponding to human MMP-12 near the N-terminus.

Epitope: N-terminus

Linkage

Replaces: AB19052

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot using a concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line (Cat. No. AG902) and probing with 1 µg/mL of Anti-MMP-12.

Western Blot Analysis: 1 µg/ml of this antibody detected MMP-12 on 10 µg of lysate.

Specificity

This antibody recognizes MMP-12 near the N-terminus of the active form.

Sequence homology to mouse is 84%.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

The zymogen for MMP-12 is ~ 54 kDa, and is quickly activated to a 45 kDa form. This breaks down to a cascade of active forms, ending with the common 22 kDa form.